Structural highlights
Publication Abstract from PubMed
Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a noncatalytic protein scaffold, we recently generated a new RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely new structure with substantially enhanced conformational dynamics, demonstrating that a primordial fold with suitable flexibility is sufficient to carry out enzymatic function.
Structure and dynamics of a primordial catalytic fold generated by in vitro evolution.,Chao FA, Morelli A, Iii JC, Churchfield L, Hagmann LN, Shi L, Masterson LR, Sarangi R, Veglia G, Seelig B Nat Chem Biol. 2012 Dec 9. doi: 10.1038/nchembio.1138. PMID:23222886[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chao FA, Morelli A, Iii JC, Churchfield L, Hagmann LN, Shi L, Masterson LR, Sarangi R, Veglia G, Seelig B. Structure and dynamics of a primordial catalytic fold generated by in vitro evolution. Nat Chem Biol. 2012 Dec 9. doi: 10.1038/nchembio.1138. PMID:23222886 doi:http://dx.doi.org/10.1038/nchembio.1138
