Structural highlights
Publication Abstract from PubMed
Carotenoid metabolism is critical for diverse physiological processes. The nematode Caenorhabditis elegans has two genes that are annotated as beta-carotene 15,15'-monooxygenase (BCMO) and are 17 centimorgan apart on chromosome II, but the function of BCMO-1 and BCMO-2 remains uncharacterized. Sequence homology indicates that the two enzymes belong to the carotenoid cleavage dioxygenase family that share a seven-bladed beta-propeller fold with a nonheme iron center. Here we determined crystal structures of BCMO-1 and BCMO-2 at resolutions of 1.8 and 1.9 A, respectively. Structural analysis reveals that BCMO-1 and BCMO-2 are strikingly similar to each other. We also characterized their beta-carotene cleavage activity, but the results suggest that they may not act as beta-carotene 15,15'-oxygenases.
Structural and Functional Analysis of Nonheme Iron Enzymes BCMO-1 and BCMO-2 from Caenorhabditis elegans.,Pan W, Zhou YL, Wang J, Dai HE, Wang X, Liu L Front Mol Biosci. 2022 Feb 10;9:844453. doi: 10.3389/fmolb.2022.844453., eCollection 2022. PMID:35223999[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pan W, Zhou YL, Wang J, Dai HE, Wang X, Liu L. Structural and Functional Analysis of Nonheme Iron Enzymes BCMO-1 and BCMO-2 from Caenorhabditis elegans. Front Mol Biosci. 2022 Feb 10;9:844453. doi: 10.3389/fmolb.2022.844453., eCollection 2022. PMID:35223999 doi:http://dx.doi.org/10.3389/fmolb.2022.844453
