1p5u
From Proteopedia
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| , resolution 1.99Å | |||||||
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| Gene: | CAF1M (Yersinia pestis), CAF1 (Yersinia pestis) | ||||||
| Related: | 1P5V
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray structure of the ternary Caf1M:Caf1:Caf1 chaperone:subunit:subunit complex
Overview
Most gram-negative pathogens express fibrous adhesive virulence organelles that mediate targeting to the sites of infection. The F1 capsular antigen from the plague pathogen Yersinia pestis consists of linear fibers of a single subunit (Caf1) and serves as a prototype for nonpilus organelles assembled via the chaperone/usher pathway. Genetic data together with high-resolution X-ray structures corresponding to snapshots of the assembly process reveal the structural basis of fiber formation. Comparison of chaperone bound Caf1 subunit with the subunit in the fiber reveals a novel type of conformational change involving the entire hydrophobic core of the protein. The observed conformational change suggests that the chaperone traps a high-energy folding intermediate of Caf1. A model is proposed in which release of the subunit allows folding to be completed, driving fiber formation.
About this Structure
1P5U is a Protein complex structure of sequences from Yersinia pestis. Full crystallographic information is available from OCA.
Reference
Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation., Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD, Cell. 2003 May 30;113(5):587-96. PMID:12787500
Page seeded by OCA on Sun Mar 30 22:55:17 2008
