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1pp4

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Revision as of 20:02, 30 March 2008 by OCA (Talk | contribs)
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PDB ID 1pp4

Drag the structure with the mouse to rotate
, resolution 2.5Å
Ligands:
Gene: RHA1 (Aspergillus aculeatus)
Related: 1DEO, 1DEX, 1K7C


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



The crystal structure of rhamnogalacturonan acetylesterase in space group P3121


Overview

The glycoprotein rhamnogalacturonan acetylesterase from Aspergillus aculeatus has been crystallized in two crystal forms, an orthorhombic and a trigonal crystal form. In the orthorhombic crystal form, the covalently bound carbohydrate at one of the two N-glycosylation sites is involved in crystal contacts. The orthorhombic crystal form was obtained at pH 5.0 and the trigonal crystal form at pH 4.5. In one case, the two crystal forms were found in the same drop at pH 4.7. The differences in crystal packing in the two crystal forms can be explained by the pH-dependent variation in the protonation state of the glutamic acid residues on the protein surface.

About this Structure

1PP4 is a Single protein structure of sequence from Aspergillus aculeatus. Full crystallographic information is available from OCA.

Reference

Crystal packing in two pH-dependent crystal forms of rhamnogalacturonan acetylesterase., Molgaard A, Larsen S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):472-8. Epub 2004, Feb 25. PMID:14993671

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