| Structural highlights
6s29 is a 4 chain structure with sequence from Cbs 356 and Fission yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | |
| Gene: | mis16, hat2, SPCC1672.10 (CBS 356), mis19, eic1, kis1, SPBC27B12.02, SPBC30B4.10 (Fission yeast) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[HAT2_SCHPO] Regulatory subunit of the histone acetylase B (HAT-B) complex (By similarity). The complex acetylates 'Lys-12' of histone H4 which is required for telomeric silencing (By similarity). Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:15369671, PubMed:24774534, PubMed:24789708, PubMed:25375240). Maintains the deacetylated state of histones specifically in the central core of the centromeres (PubMed:15369671).[UniProtKB:P39984][1] [2] [3] [4] [MIS19_SCHPO] Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:24774534, PubMed:24789708, PubMed:25375240). Links mis16 and mis18 to recruit CENP-A through interacting with non-sense-mediated mRNA decay (NMD) factors and the SWI/SNF complex (PubMed:24774534). Links also mis18 with the CCAN/mis6/ctf19 complex to promote CENP-A assembly (PubMed:24789708).[5] [6] [7]
Publication Abstract from PubMed
Centromeric chromatin in fission yeast is distinguished by the presence of nucleosomes containing the histone H3 variant Cnp1(CENP-A) Cell cycle-specific deposition of Cnp1 requires the Mis16-Mis18-Mis19 complex, which is thought to direct recruitment of Scm3-chaperoned Cnp1/histone H4 dimers to DNA. Here, we present the structure of the essential Mis18 partner protein Mis19 and describe its interaction with Mis16, revealing a bipartite-binding site. We provide data on the stoichiometry and overall architecture of the complex and provide detailed insights into the Mis18-Mis19 interface.
Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex.,Korntner-Vetter M, Lefevre S, Hu XW, George R, Singleton MR Life Sci Alliance. 2019 Aug 1;2(4). pii: 2/4/e201900408. doi:, 10.26508/lsa.201900408. Print 2019 Aug. PMID:31371524[8]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hayashi T, Fujita Y, Iwasaki O, Adachi Y, Takahashi K, Yanagida M. Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Cell. 2004 Sep 17;118(6):715-29. PMID:15369671 doi:http://dx.doi.org/10.1016/j.cell.2004.09.002
- ↑ Hayashi T, Ebe M, Nagao K, Kokubu A, Sajiki K, Yanagida M. Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex. Genes Cells. 2014 Jul;19(7):541-54. doi: 10.1111/gtc.12152. Epub 2014 Apr 29. PMID:24774534 doi:http://dx.doi.org/10.1111/gtc.12152
- ↑ Subramanian L, Toda NR, Rappsilber J, Allshire RC. Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. Open Biol. 2014 Apr 30;4:140043. doi: 10.1098/rsob.140043. PMID:24789708 doi:http://dx.doi.org/10.1098/rsob.140043
- ↑ Hirai H, Arai K, Kariyazono R, Yamamoto M, Sato M. The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A. PLoS One. 2014 Nov 6;9(11):e111905. doi: 10.1371/journal.pone.0111905., eCollection 2014. PMID:25375240 doi:http://dx.doi.org/10.1371/journal.pone.0111905
- ↑ Hayashi T, Ebe M, Nagao K, Kokubu A, Sajiki K, Yanagida M. Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex. Genes Cells. 2014 Jul;19(7):541-54. doi: 10.1111/gtc.12152. Epub 2014 Apr 29. PMID:24774534 doi:http://dx.doi.org/10.1111/gtc.12152
- ↑ Subramanian L, Toda NR, Rappsilber J, Allshire RC. Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. Open Biol. 2014 Apr 30;4:140043. doi: 10.1098/rsob.140043. PMID:24789708 doi:http://dx.doi.org/10.1098/rsob.140043
- ↑ Hirai H, Arai K, Kariyazono R, Yamamoto M, Sato M. The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A. PLoS One. 2014 Nov 6;9(11):e111905. doi: 10.1371/journal.pone.0111905., eCollection 2014. PMID:25375240 doi:http://dx.doi.org/10.1371/journal.pone.0111905
- ↑ Korntner-Vetter M, Lefevre S, Hu XW, George R, Singleton MR. Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex. Life Sci Alliance. 2019 Aug 1;2(4). pii: 2/4/e201900408. doi:, 10.26508/lsa.201900408. Print 2019 Aug. PMID:31371524 doi:http://dx.doi.org/10.26508/lsa.201900408
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