Structural highlights
Function
[BIKB_THET8] Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide (By similarity). Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate. It also converts 2-amino-3-ketobutyrate and CoA to glycine and acetyl-CoA. Activity is also observed with the following combinations of substrates: acetyl-CoA and either L-alanine or L-serine, pimeloyl-CoA and either glycine or L-serine, and palmitoyl-CoA with L-alanine.[1]
References
- ↑ Kubota T, Shimono J, Kanameda C, Izumi Y. The first thermophilic alpha-oxoamine synthase family enzyme that has activities of 2-amino-3-ketobutyrate CoA ligase and 7-keto-8-aminopelargonic acid synthase: cloning and overexpression of the gene from an extreme thermophile, Thermus thermophilus, and characterization of its gene product. Biosci Biotechnol Biochem. 2007 Dec;71(12):3033-40. doi: 10.1271/bbb.70438. Epub , 2007 Dec 7. PMID:18071260 doi:http://dx.doi.org/10.1271/bbb.70438
