Structural highlights
Function
[AZUP_ACHCY] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress.
Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013.,Inoue T, Nishio N, Kai Y, Harada S, Ohshiro Y, Suzuki S, Kohzuma T, Shidara S, Iwasaki H J Biochem. 1993 Dec;114(6):761-2. PMID:8138527[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Inoue T, Nishio N, Kai Y, Harada S, Ohshiro Y, Suzuki S, Kohzuma T, Shidara S, Iwasaki H. Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013. J Biochem. 1993 Dec;114(6):761-2. PMID:8138527
