1qfc
From Proteopedia
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| , resolution 2.70Å | |||||||
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| Ligands: | , , | ||||||
| Activity: | Acid phosphatase, with EC number 3.1.3.2 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF RAT PURPLE ACID PHOSPHATASE
Overview
Tartrate-resistant acid phosphatase (TRAP) is a mammalian di-iron- containing enzyme that belongs to the family of purple acid phosphatases (PAP). It is highly expressed in a limited number of tissues, predominantly in bone-resorbing osteoclasts and in macrophages of spleen. We have determined the crystal structure of rat TRAP in complex with a phosphate ion to 2.7 A resolution. The fold resembles that of the catalytic domain of kidney bean purple acid phosphatase (KBPAP), although the sequence similarity is limited to the active site residues. A surface loop near the active site is absent due to proteolysis, leaving the active-site easily accessible from the surrounding solvent. This, we believe, gives a structural explanation for the observed proteolytic activation of TRAP. The current structure was determined at a relatively high pH and without any external reducing agents. It is likely that it represents an oxidized and therefore catalytically inactive form of the enzyme.
About this Structure
1QFC is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a mammalian purple acid phosphatase., Uppenberg J, Lindqvist F, Svensson C, Ek-Rylander B, Andersson G, J Mol Biol. 1999 Jul 2;290(1):201-11. PMID:10388567
Page seeded by OCA on Sun Mar 30 23:12:40 2008
