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Publication Abstract from PubMed

Binding isotope effects for l-aspartate reacting with the inactive K258A mutant of PLP-dependent aspartate aminotransferase to give a stable external aldimine intermediate are reported. They provide direct evidence for electronic ground-state destabilization via hyperconjugation. The smaller equilibrium isotope effect with deazaPLP-reconstituted K258A indicates that the pyridine nitrogen plays an important role in labilizing the Calpha-H bond.

Ground-state electronic destabilization via hyperconjugation in aspartate aminotransferase.,Griswold WR, Castro JN, Fisher AJ, Toney MD J Am Chem Soc. 2012 May 23;134(20):8436-8. doi: 10.1021/ja302809e. Epub 2012 May , 10. PMID:22551424^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.