4xdn
From Proteopedia
Crystal structure of Scc4 in complex with Scc2n
Structural highlights
FunctionSCC4_YEAST Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.[1] Publication Abstract from PubMedThe cohesin ring holds newly replicated sister chromatids together until their separation at anaphase. Initiation of sister chromatid cohesion depends on a separate complex, Scc2NIPBL/Scc4Mau2 (Scc2/4), which loads cohesin onto DNA and determines its localization across the genome. Proper cohesin loading is essential for cell division, and partial defects cause chromosome missegregation and aberrant transcriptional regulation, leading to severe developmental defects in multicellular organisms. We present here a crystal structure showing the interaction between Scc2 and Scc4. Scc4 is a TPR array that envelops an extended Scc2 peptide. Using budding yeast, we demonstrate that a conserved patch on the surface of Scc4 is required to recruit Scc2/4 to centromeres and to build pericentromeric cohesion. These findings reveal the role of Scc4 in determining the localization of cohesin loading and establish a molecular basis for Scc2/4 recruitment to centromeres. Structural evidence for Scc4-dependent localization of cohesin loading.,Hinshaw SM, Makrantoni V, Kerr A, Marston AL, Harrison SC Elife. 2015 Jun 3;4. doi: 10.7554/eLife.06057. PMID:26038942[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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