| Structural highlights
Function
[ROQ1_NICBE] Disease resistance (R) protein that specifically recognizes the Xanthomonas and Pseudomonas effector proteins XopQ and HopQ1, and triggers cell death (PubMed:28891100). Acts as a NAD(+) hydrolase (NADase): in response to activation, catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR) and nicotinamide; NAD(+) cleavage triggering a defense system that promotes cell death (PubMed:31439792).[1] [2]
Publication Abstract from PubMed
Plants and animals detect pathogen infection using intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) that directly or indirectly recognize pathogen effectors and activate an immune response. How effector sensing triggers NLR activation remains poorly understood. Here we describe the 3.8-angstrom-resolution cryo-electron microscopy structure of the activated ROQ1 (recognition of XopQ 1), an NLR native to Nicotiana benthamiana with a Toll-like interleukin-1 receptor (TIR) domain bound to the Xanthomonas euvesicatoria effector XopQ (Xanthomonas outer protein Q). ROQ1 directly binds to both the predicted active site and surface residues of XopQ while forming a tetrameric resistosome that brings together the TIR domains for downstream immune signaling. Our results suggest a mechanism for the direct recognition of effectors by NLRs leading to the oligomerization-dependent activation of a plant resistosome and signaling by the TIR domain.
Structure of the activated ROQ1 resistosome directly recognizing the pathogen effector XopQ.,Martin R, Qi T, Zhang H, Liu F, King M, Toth C, Nogales E, Staskawicz BJ Science. 2020 Dec 4;370(6521). pii: 370/6521/eabd9993. doi:, 10.1126/science.abd9993. PMID:33273074[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schultink A, Qi T, Lee A, Steinbrenner AD, Staskawicz B. Roq1 mediates recognition of the Xanthomonas and Pseudomonas effector proteins XopQ and HopQ1. Plant J. 2017 Dec;92(5):787-795. doi: 10.1111/tpj.13715. Epub 2017 Oct 25. PMID:28891100 doi:http://dx.doi.org/10.1111/tpj.13715
- ↑ Horsefield S, Burdett H, Zhang X, Manik MK, Shi Y, Chen J, Qi T, Gilley J, Lai JS, Rank MX, Casey LW, Gu W, Ericsson DJ, Foley G, Hughes RO, Bosanac T, von Itzstein M, Rathjen JP, Nanson JD, Boden M, Dry IB, Williams SJ, Staskawicz BJ, Coleman MP, Ve T, Dodds PN, Kobe B. NAD(+) cleavage activity by animal and plant TIR domains in cell death pathways. Science. 2019 Aug 23;365(6455):793-799. doi: 10.1126/science.aax1911. PMID:31439792 doi:http://dx.doi.org/10.1126/science.aax1911
- ↑ Martin R, Qi T, Zhang H, Liu F, King M, Toth C, Nogales E, Staskawicz BJ. Structure of the activated ROQ1 resistosome directly recognizing the pathogen effector XopQ. Science. 2020 Dec 4;370(6521). pii: 370/6521/eabd9993. doi:, 10.1126/science.abd9993. PMID:33273074 doi:http://dx.doi.org/10.1126/science.abd9993
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