6e3e
From Proteopedia
Structure of RORgt in complex with a novel inverse agonist.
Structural highlights
Function[RORG_HUMAN] Possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivators recruitment. Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. Involved in lineage specification of uncommitted CD4(+) T-helper cells into Th17 cells. Regulate the expression of several components of the circadian clock. Publication Abstract from PubMedRetinoic acid receptor-related orphan receptor gammat (RORgammat) agonists are expected to provide a novel class of immune-activating anticancer drugs via activation of Th17 cells and Tc17 cells. Herein, we describe a novel structure-based functionality switching approach from in house well-optimized RORgammat inverse agonists to potent RORgammat agonists. We succeeded in the identification of potent RORgammat agonist 5 without major chemical structure change. The biochemical response was validated by molecular dynamics simulation studies that showed a helix 12 stabilization effect of RORgammat agonists. These results indicate that targeting helix 12 is an attractive and novel medicinal chemistry strategy for switching existing RORgammat inverse agonists to agonists. Design, Synthesis, and Biological Evaluation of Retinoic Acid-Related Orphan Receptor gammat (RORgammat) Agonist Structure-Based Functionality Switching Approach from In House RORgammat Inverse Agonist to RORgammat Agonist.,Yukawa T, Nara Y, Kono M, Sato A, Oda T, Takagi T, Sato T, Banno Y, Taya N, Imada T, Shiokawa Z, Negoro N, Kawamoto T, Koyama R, Uchiyama N, Skene R, Hoffman I, Chen CH, Sang B, Snell G, Katsuyama R, Yamamoto S, Shirai J J Med Chem. 2019 Feb 14;62(3):1167-1179. doi: 10.1021/acs.jmedchem.8b01181. Epub , 2019 Feb 4. PMID:30652849[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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