4dou
From Proteopedia
Crystal Structure of a Single-chain Trimer of Human Adiponectin Globular Domain
Structural highlights
Disease[ADIPO_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. Disease susceptibility is associated with variations affecting the gene represented in this entry. Function[ADIPO_HUMAN] Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.[1] Publication Abstract from PubMedAdiponectin is increasingly recognized as a potential therapeutic agent for the treatment of diabetes and other metabolic diseases. It circulates in plasma as homotrimers and higher-order oliogomers of homotrimers. To facilitate the production of active recombinant adiponectin as a therapeutic tool, we designed a single-chain globular domain adiponectin (sc-gAd) in which three monomer sequences are linked together in tandem to form one contiguous polypeptide. Here, we present the crystal structure of human sc-gAd at 2.0A resolution. The structure reveals a similar trimeric topology to that of mouse gAd protein. Trimer formation is further rigidified by three calcium ions. Crystal structure of a single-chain trimer of human adiponectin globular domain.,Min X, Lemon B, Tang J, Liu Q, Zhang R, Walker N, Li Y, Wang Z FEBS Lett. 2012 Mar 23;586(6):912-7. Epub 2012 Feb 22. PMID:22449980[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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