| Structural highlights
Function
CSM2_YEAST Involved in chromosome segregation during meiosis. Promotes efficient recombinational repair and functions in the protection of the genome from spontaneous and induced DNA damage like mutations and gross chromosomal rearrangements (GCRs).[1] [2] [3] [4] [5]
Publication Abstract from PubMed
In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8A resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS:: PSY3 and CSM2bind by X-ray crystallography (View interaction) PSY3, CSM2, Shu 1 and Shu 2physically interact by x ray scattering (View interaction).
Structural and SAXS analysis of the budding yeast SHU-complex proteins.,She Z, Gao ZQ, Liu Y, Wang WJ, Liu GF, Shtykova EV, Xu JH, Dong YH FEBS Lett. 2012 Jul 30;586(16):2306-12. Epub 2012 Jun 27. PMID:22749910[6]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rabitsch KP, Toth A, Galova M, Schleiffer A, Schaffner G, Aigner E, Rupp C, Penkner AM, Moreno-Borchart AC, Primig M, Esposito RE, Klein F, Knop M, Nasmyth K. A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol. 2001 Jul 10;11(13):1001-9. PMID:11470404
- ↑ Huang ME, Rio AG, Nicolas A, Kolodner RD. A genomewide screen in Saccharomyces cerevisiae for genes that suppress the accumulation of mutations. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11529-34. Epub 2003 Sep 12. PMID:12972632 doi:http://dx.doi.org/10.1073/pnas.2035018100
- ↑ Smith S, Hwang JY, Banerjee S, Majeed A, Gupta A, Myung K. Mutator genes for suppression of gross chromosomal rearrangements identified by a genome-wide screening in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):9039-44. Epub 2004 Jun 7. PMID:15184655 doi:10.1073/pnas.0403093101
- ↑ Shor E, Weinstein J, Rothstein R. A genetic screen for top3 suppressors in Saccharomyces cerevisiae identifies SHU1, SHU2, PSY3 and CSM2: four genes involved in error-free DNA repair. Genetics. 2005 Mar;169(3):1275-89. Epub 2005 Jan 16. PMID:15654096 doi:10.1534/genetics.104.036764
- ↑ Ball LG, Zhang K, Cobb JA, Boone C, Xiao W. The yeast Shu complex couples error-free post-replication repair to homologous recombination. Mol Microbiol. 2009 Jul;73(1):89-102. Epub 2009 Jun 1. PMID:19496932 doi:MMI6748
- ↑ She Z, Gao ZQ, Liu Y, Wang WJ, Liu GF, Shtykova EV, Xu JH, Dong YH. Structural and SAXS analysis of the budding yeast SHU-complex proteins. FEBS Lett. 2012 Jul 30;586(16):2306-12. Epub 2012 Jun 27. PMID:22749910 doi:10.1016/j.febslet.2012.06.024
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