5ydg
From Proteopedia
Crystal structure of the Arabidopsis thaliana chloroplast RNA editing factors 2(MORF2)
Structural highlights
Function[MORF2_ARATH] Involved in plastid rRNA processing and consequently in translation and early chloroplast differentiation (PubMed:12678554). Involved in organellar RNA editing. Required for the processing of multiple editing sites in plastids (PubMed:22411807, PubMed:23818871).[1] [2] [3] Publication Abstract from PubMedRNA editing is a post-transcription process that alters the genetic information on RNA molecules. In plastids and mitochondria of flowering plants, the multiple organellar RNA editing factors (MORFs) interact with the PLS-type pentatricopeptide repeat (PPR) proteins and participate in RNA editing of cytidine-to-uridine conversion. The PPR proteins recognize cytidine targets around the editing sites, and the MORF proteins modulate the RNA-binding activity of the PPR proteins. Here, we report the structure of the Arabidopsis thaliana chloroplast MORF2 at 2.4 A resolution. The structure, adopting typical MORF-box fold as observed in mitochondrial MORF1 and chloroplast MORF9, reveals an MORF1-like dimerization mode. The difference between the two dimerization modes can be attributed to F157 (corresponding F162 in MORF1 and W160 in MORF9), which causes a 60 degrees shift upon dimerization. This observation, together with the PPR-MORF2 model, suggests a dimer-to-monomer transition during RNA editosome formation. Crystal structure of the chloroplast RNA editing factor MORF2.,Yang J, Zhang M, Wang X Biochem Biophys Res Commun. 2017 Dec 8. pii: S0006-291X(17)32435-X. doi:, 10.1016/j.bbrc.2017.12.044. PMID:29229384[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Arath | Gao, Y S | Wang, X | Wang, Y L | Yang, J Y | Arabidopsis thaliana | Morf | Rna binding protein | Rna editing
