6jdp

Publication Abstract from PubMed

The bacterial type VI secretion system (T6SS) utilizes many toxic effectors to gain advantage over inter-bacterial competition and eukaryotic host infection. Meanwhile, the cognate immunity proteins of these effectors are employed to protect themselves from the virulence. TseT and TsiT form an effector-immunity (E-I) protein pair secreted by T6SS of Pseudomonas aeruginosa. TseT is toxic for other bacteria, whereas TsiT can suppress the virulence of TseT. Here, we report the crystal structure of TsiT at 1.6 a resolution. TsiT is a typical alpha+beta class protein and belongs to a novel Imm52 protein family of the polymorphic toxin system. Apart from TsiT, only one structure of the Imm52 family proteins is present in the Protein Data Bank (PDB), but that structure is not characterized and shares low sequence identity with TsiT. We characterized the basic features of TsiT structure and identified conserved residues of the Imm52 family proteins according to homology comparison. Our work provided structural information of a new protein family and should aid future functional studies. This article is protected by copyright. All rights reserved.

Structural characterization of the Imm52 family protein TsiT in Pseudomonas aeruginosa.,She Z, Geng Z, Xu JH, Li YH, Dong YH Protein Sci. 2019 Mar 4. doi: 10.1002/pro.3597. PMID:30834616^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.