6jle
From Proteopedia
Crystal structure of MORN4/Myo3a complex
Structural highlights
Disease[MYO3A_HUMAN] Autosomal recessive non-syndromic sensorineural deafness type DFNB. The disease is caused by mutations affecting the gene represented in this entry. Function[MORN4_MOUSE] Plays a role in promoting axonal degeneration following neuronal injury by toxic insult or trauma.[1] [MYO3A_HUMAN] Probable actin-based motor with a protein kinase activity. Probably plays a role in vision and hearing (PubMed:12032315). Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern. Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (By similarity).[UniProtKB:Q8K3H5][2] Publication Abstract from PubMedTandem repeats are basic building blocks for constructing proteins with diverse structures and functions. Compared with extensively studied alpha-helix-based tandem repeats such as ankyrin, tetratricopeptide, armadillo, and HEAT repeat proteins, relatively little is known about tandem repeat proteins formed by beta hairpins. In this study, we discovered that the MORN repeats from MORN4 function as a protein binding module specifically recognizing a tail cargo binding region from Myo3a. The structure of the MORN4/Myo3a complex shows that MORN4 forms an extended single-layered beta-sheet structure and uses a U-shaped groove to bind to the Myo3a tail with high affinity and specificity. Sequence and structural analyses further elucidated the unique sequence features for folding and target binding of MORN repeats. Our work establishes that the beta-hairpin-based MORN repeats are protein-protein interaction modules. Structure of the MORN4/Myo3a Tail Complex Reveals MORN Repeats as Protein Binding Modules.,Li J, Liu H, Raval MH, Wan J, Yengo CM, Liu W, Zhang M Structure. 2019 Jun 28. pii: S0969-2126(19)30203-5. doi:, 10.1016/j.str.2019.06.004. PMID:31279628[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||||||
