Structural highlights
7dag is a 8 chain structure with sequence from "bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Gene: | adhE_1, adhC, adhE, adhE_2, BC353_06450, C9J66_00760, D6U80_02570, ERS013138_00532, ERS013165_00179, ERS013186_01240, ERS013198_03108, ERS013199_01145, ERS013201_00485, ERS013202_01518, ERS013206_03377, FLM02_10510, FXF03_12520 ("Bacillo virgola del Koch" Trevisan 1884) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Aldehyde-alcohol dehydrogenase (AdhE) is a metabolic enzyme and virulence factor in bacteria. E. coli AdhE (eAdhE) multimerizes into spirosomes that are essential for enzymatic activity. However, it is unknown whether AdhE structure is conserved in divergent bacteria. Here, we present the cryo-EM structure of AdhE (vAdhE) from Vibrio cholerae to 4.31 A resolution. Overall, vAdhE spirosomes are similar to eAdhE with conserved subunit arrangement. However, divergences in key oligomerization residues cause vAdhE to form labile spirosomes with lower enzymatic activity. Mutating the vAdhE oligomerization interface to mimic eAdhE increases spirosome stability and enzymatic activity to levels comparable to eAdhE. These results support the generality of AdhE spirosome structures, and provide a structural basis to target vAdhE to attenuate bacterial virulence.
Cryo-EM structure of Vibrio cholerae aldehyde-alcohol dehydrogenase spirosomes.,Cho S, Kim G, Song JJ, Cho C Biochem Biophys Res Commun. 2021 Jan 15;536:38-44. doi:, 10.1016/j.bbrc.2020.12.040. Epub 2020 Dec 23. PMID:33360541[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cho S, Kim G, Song JJ, Cho C. Cryo-EM structure of Vibrio cholerae aldehyde-alcohol dehydrogenase spirosomes. Biochem Biophys Res Commun. 2021 Jan 15;536:38-44. doi:, 10.1016/j.bbrc.2020.12.040. Epub 2020 Dec 23. PMID:33360541 doi:http://dx.doi.org/10.1016/j.bbrc.2020.12.040
