1nj3
From Proteopedia
Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4
Structural highlights
Function[NPL4_RAT] The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.[1] [2] [3] [4] [5] [6] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedUbiquitylated proteins are directed into a large number of different cellular pathways through interactions with effector proteins that contain conserved ubiquitin binding motifs. Here, we report the solution structure and ubiquitin binding properties of one such motif, the Npl4 zinc finger or RanBP2/Nup358 zinc finger (NZF) domain. Npl4 NZF forms a compact module composed of four antiparallel beta-strands linked by three ordered loops. A single zinc ion is coordinated by four conserved cysteines from the first and third loops, which form two rubredoxin knuckles. Npl4 NZF binds specifically, but weakly, to free ubiquitin using a conserved 13TF14 dipeptide to interact with the "Ile-44" surface of ubiquitin. Our studies reveal the structure of this versatile class of protein binding domains and provide a means for identifying the subset of NZF domains likely to bind ubiquitin. Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4.,Wang B, Alam SL, Meyer HH, Payne M, Stemmler TL, Davis DR, Sundquist WI J Biol Chem. 2003 May 30;278(22):20225-34. Epub 2003 Mar 18. PMID:12644454[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Buffalo rat | Large Structures | Alam, S L | Davis, D R | Meyer, H H | Payne, M | Stemmler, T L | Sundquist, W I | Wang, B | Beta-ribbon | Npl4 | Nzf domain | Protein binding | Rubredoxin knuckle | Ubiquitin | Zinc-finger
