Structural highlights
Publication Abstract from PubMed
A hyperstable Pin1 WW domain has been circularly permuted via excision of the fold-nucleating turn; it still folds to form the native three-strand sheet and hydrophobic core features. Multiprobe folding dynamics studies of the normal and circularly permuted sequences, as well as their constituent hairpin fragments and comparable-length beta-strand-loop-beta-strand models, indicate 2-state folding for all topologies. N-terminal hairpin formation is the fold nucleating event for the wild-type sequence; the slower folding circular permutant has a more distributed folding transition state.
Circular Permutation of a WW Domain: Folding Still Occurs after Excising the Turn of the Folding-Nucleating Hairpin.,Kier BL, Anderson JM, Andersen NH J Am Chem Soc. 2014 Jan 3. PMID:24350581[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kier BL, Anderson JM, Andersen NH. Circular Permutation of a WW Domain: Folding Still Occurs after Excising the Turn of the Folding-Nucleating Hairpin. J Am Chem Soc. 2014 Jan 3. PMID:24350581 doi:http://dx.doi.org/10.1021/ja410824x
