6tjp
From Proteopedia
Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve - P212121
Structural highlights
Function[PORTL_BPT7] Forms the portal vertex of the capsid. This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel.[HAMAP-Rule:MF_04120][1] [2] Publication Abstract from PubMedMedium-resolution cryo-electron microscopy maps, in particular when they include a significant number of alpha-helices, may allow the building of partial models that are useful for molecular-replacement searches in large crystallographic structures when the structures of homologs are not available and experimental phasing has failed. Here, as an example, the solution of the structure of a bacteriophage portal using a partial 30% model built into a 7.8 A resolution cryo-EM map is shown. Inspection of the self-rotation function allowed the correct oligomerization state to be determined, and density-modification procedures using rotation matrices and a mask based on the cryo-EM structure were critical for solving the structure. A workflow is described that may be applicable to similar cases and this strategy is compared with direct use of the cryo-EM map for molecular replacement. Using a partial atomic model from medium-resolution cryo-EM to solve a large crystal structure.,Fabrega-Ferrer M, Cuervo A, Fernandez FJ, Machon C, Perez-Luque R, Pous J, Vega MC, Carrascosa JL, Coll M Acta Crystallogr D Struct Biol. 2021 Jan 1;77(Pt 1):11-18. doi:, 10.1107/S2059798320015156. Epub 2021 Jan 1. PMID:33404521[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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