Structural highlights
Publication Abstract from PubMed
The enzyme ForT catalyzes C-C bond formation between 5'-phosphoribosyl-1'-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5'-phosphate by Streptomyces kaniharaensis. We report the 2.5 A resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.
Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex.,Gao S, Radadiya A, Li W, Liu H, Zhu W, de Crecy-Lagard V, Richards NGJ, Naismith JH Chem Commun (Camb). 2020 Jul 14;56(55):7617-7620. doi: 10.1039/d0cc02834g. Epub, 2020 Jun 9. PMID:32515440[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gao S, Radadiya A, Li W, Liu H, Zhu W, de Crecy-Lagard V, Richards NGJ, Naismith JH. Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex. Chem Commun (Camb). 2020 Jul 14;56(55):7617-7620. doi: 10.1039/d0cc02834g. Epub, 2020 Jun 9. PMID:32515440 doi:http://dx.doi.org/10.1039/d0cc02834g
