8ka4

Structural highlights

Function

ARP_ARATH Repairs oxidative DNA damages, seems also to act as a redox factor (PubMed:7512729). Is multifunctional and may be involved both in DNA repair and in the regulation of transcription (PubMed:7512729). Exhibits apurinic/apyrimidinic (AP) endonuclease activity (PubMed:25569774, PubMed:21781197, PubMed:25228464). Catalyzes the conversion of 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) to 3'-OH (PubMed:25228464). May be involved in base excision repair in chloroplasts (PubMed:19372224). According to a report, has a significant in vitro 3'-phosphatase activity (PubMed:25228464). According to another report, has no in vitro 3'-phosphatase activity (PubMed:25569774). Has a strong non-specific affinity to DNA (PubMed:25228464).^{[1] [2] [3] [4] [5] [6]}

References

1. ↑ Gutman BL, Niyogi KK. Evidence for base excision repair of oxidative DNA damage in chloroplasts of Arabidopsis thaliana. J Biol Chem. 2009 Jun 19;284(25):17006-17012. PMID:19372224 doi:10.1074/jbc.M109.008342
2. ↑ Córdoba-Cañero D, Roldán-Arjona T, Ariza RR. Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1. Plant J. 2011 Nov;68(4):693-702. PMID:21781197 doi:10.1111/j.1365-313X.2011.04720.x
3. ↑ Lee J, Jang H, Shin H, Choi WL, Mok YG, Huh JH. AP endonucleases process 5-methylcytosine excision intermediates during active DNA demethylation in Arabidopsis. Nucleic Acids Res. 2014 Oct;42(18):11408-18. doi: 10.1093/nar/gku834. Epub 2014 , Sep 16. PMID:25228464 doi:http://dx.doi.org/10.1093/nar/gku834
4. ↑ Li Y, Córdoba-Cañero D, Qian W, Zhu X, Tang K, Zhang H, Ariza RR, Roldán-Arjona T, Zhu JK. An AP endonuclease functions in active DNA demethylation and gene imprinting in Arabidopsis [corrected]. PLoS Genet. 2015 Jan 8;11(1):e1004905. PMID:25569774 doi:10.1371/journal.pgen.1004905
5. ↑ Babiychuk E, Kushnir S, Van Montagu M, Inzé D. The Arabidopsis thaliana apurinic endonuclease Arp reduces human transcription factors Fos and Jun. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3299-303. PMID:7512729 doi:10.1073/pnas.91.8.3299
6. ↑ Murphy TM, Belmonte M, Shu S, Britt AB, Hatteroth J. Requirement for abasic endonuclease gene homologues in Arabidopsis seed development. PLoS One. 2009;4(1):e4297. PMID:19172180 doi:10.1371/journal.pone.0004297