Structural highlights
Function
A0A640KC69_LEITA
Publication Abstract from PubMed
Propionyl-CoA carboxylase (PCC) is a multienzyme complex consisting of up to six alpha-subunits and six beta-subunits. Belonging to a metabolic pathway converging on the citric acid cycle, it is present in most forms of life and irregularities in its assembly lead to serious illness in humans, known as propionic acidemia. Here, we report the cryogenic electron microscopy (cryoEM) structures and assembly of different oligomeric isomers of endogenous PCC from the parasitic protozoan Leishmania tarentolae (LtPCC). These structures and their statistical distribution reveal the mechanics of PCC assembly and disassembly at equilibrium. We show that, in solution, endogenous LtPCC beta-subunits form stable homohexamers, to which different numbers of alpha-subunits attach. Sorting LtPCC particles into seven classes (i.e., oligomeric formulae alpha(0)beta(6), alpha(1)beta(6), alpha(2)beta(6), alpha(3)beta(6), alpha(4)beta(6), alpha(5)beta(6), alpha(6)beta(6)) enables formulation of a model for PCC assembly. Our results suggest how multimerization regulates PCC enzymatic activity and showcase the utility of cryoEM in revealing the statistical mechanics of reaction pathways.
CryoEM reveals oligomeric isomers of a multienzyme complex and assembly mechanics.,Lee JKJ, Liu YT, Hu JJ, Aphasizheva I, Aphasizhev R, Zhou ZH J Struct Biol X. 2023 Apr 8;7:100088. doi: 10.1016/j.yjsbx.2023.100088. , eCollection 2023. PMID:37128595[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lee JKJ, Liu YT, Hu JJ, Aphasizheva I, Aphasizhev R, Zhou ZH. CryoEM reveals oligomeric isomers of a multienzyme complex and assembly mechanics. J Struct Biol X. 2023 Apr 8;7:100088. PMID:37128595 doi:10.1016/j.yjsbx.2023.100088
