Structural highlights
Function
PANX1_MOUSE Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis (By similarity). Structural component of the gap junctions and the hemichannels involved in the ATP release and nucleotide permeation. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis. Plays a critical role in oogenesis.[UniProtKB:Q96RD7]
Publication Abstract from PubMed
Membrane proteins are the largest group of therapeutic targets in a variety of disease areas and yet, they remain particularly difficult to investigate. We have developed a novel one-step approach for the incorporation of membrane proteins directly from cells into lipid Salipro nanoparticles. Here, with the pannexin1 channel as a case study, we demonstrate the applicability of this method for structure-function analysis using SPR and cryo-EM.
Direct cell extraction of membrane proteins for structure-function analysis.,Drulyte I, Gutgsell AR, Lloris-Garcera P, Liss M, Geschwindner S, Radjainia M, Frauenfeld J, Loving R Sci Rep. 2023 Jan 25;13(1):1420. doi: 10.1038/s41598-023-28455-w. PMID:36697499[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Drulyte I, Gutgsell AR, Lloris-Garcerá P, Liss M, Geschwindner S, Radjainia M, Frauenfeld J, Löving R. Direct cell extraction of membrane proteins for structure-function analysis. Sci Rep. 2023 Jan 25;13(1):1420. PMID:36697499 doi:10.1038/s41598-023-28455-w
