Structural highlights
Function
ADRB2_HUMAN Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Publication Abstract from PubMed
A highly crystallizable T4 lysozyme (T4L) was fused to the N-terminus of the beta(2) adrenergic receptor (beta(2)AR), a G-protein coupled receptor (GPCR) for catecholamines. We demonstrate that the N-terminal fused T4L is sufficiently rigid relative to the receptor to facilitate crystallogenesis without thermostabilizing mutations or the use of a stabilizing antibody, G protein, or protein fused to the 3rd intracellular loop. This approach adds to the protein engineering strategies that enable crystallographic studies of GPCRs alone or in complex with a signaling partner.
N-terminal t4 lysozyme fusion facilitates crystallization of a g protein coupled receptor.,Zou Y, Weis WI, Kobilka BK PLoS One. 2012;7(10):e46039. doi: 10.1371/journal.pone.0046039. Epub 2012 Oct 4. PMID:23056231[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zou Y, Weis WI, Kobilka BK. N-terminal t4 lysozyme fusion facilitates crystallization of a g protein coupled receptor. PLoS One. 2012;7(10):e46039. doi: 10.1371/journal.pone.0046039. Epub 2012 Oct 4. PMID:23056231 doi:http://dx.doi.org/10.1371/journal.pone.0046039
