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Publication Abstract from PubMed

Plant defensins demonstrate high structural stability at extreme temperatures and pH values and, in general, are non-toxic to mammalian cells. These properties make them attractive candidates for use in biotechnology and biomedicine. Knowing the structure-function relationship is desirable to guide the design of plant defensin-based applications. Thus far, the broad range of biological activities was described only for one defensin from gymnosperms, the defensin PsDef1 from Scots pine. Here, we report that closely related defensin from the same taxonomy group, PsDef2, differing from PsDef1 by six amino acids, also possesses antimicrobial, antibacterial, and insect alpha-amylase inhibitory activities. We also report the solution structure and dynamics properties of PsDef2 assessed using a combination of experimental nuclear magnetic resonance (NMR) techniques. Lastly, we perform a comparative analysis of PsDef2 and PsDef1 gaining a molecular-level insight into their structure-dynamics-function relationship.

Structure, dynamics, and function of PsDef2 defensin from Pinus sylvestris.,Bukhteeva I, Hrunyk NI, Yusypovych YM, Shalovylo YI, Kovaleva V, Nesmelova IV Structure. 2022 Mar 15. pii: S0969-2126(22)00082-X. doi:, 10.1016/j.str.2022.03.001. PMID:35334207^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.