8arf

Structural highlights

Function

[SPDLY_HUMAN] Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment (PubMed:17576797, PubMed:19468067). Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494). Plays a role in cell migration (PubMed:30258100).[HAMAP-Rule:MF_03041]^{[1] [2] [3] [4]}

References

1. ↑ Griffis ER, Stuurman N, Vale RD. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J Cell Biol. 2007 Jun 18;177(6):1005-15. PMID:17576797 doi:http://dx.doi.org/jcb.200702062
2. ↑ Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J Cell Biol. 2009 Jun 1;185(5):859-74. doi: 10.1083/jcb.200812167. Epub 2009 May , 25. PMID:19468067 doi:http://dx.doi.org/10.1083/jcb.200812167
3. ↑ McKenney RJ, Huynh W, Tanenbaum ME, Bhabha G, Vale RD. Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes. Science. 2014 Jul 18;345(6194):337-41. doi: 10.1126/science.1254198. Epub 2014, Jun 19. PMID:25035494 doi:http://dx.doi.org/10.1126/science.1254198
4. ↑ Conte C, Griffis ER, Hickson I, Perez-Oliva AB. USP45 and Spindly are part of the same complex implicated in cell migration. Sci Rep. 2018 Sep 26;8(1):14375. doi: 10.1038/s41598-018-32685-8. PMID:30258100 doi:http://dx.doi.org/10.1038/s41598-018-32685-8