5htb
From Proteopedia
Crystal structure of haspin (GSG2) in complex with bisubstrate inhibitor ARC-3353
Structural highlights
FunctionHASP_HUMAN Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.[1] [2] [3] [4] [5] Publication Abstract from PubMedHaspin is a mitotic protein kinase that is responsible for the phosphorylation of Thr3 of histone H3, thereby creating a recognition motif for docking of the chromosomal passenger complex that is crucial for the progression of cell division. Here, two high-resolution models of haspin with previously reported inhibitors consisting of an ATP analogue and a histone H3(1-7) peptide analogue are presented. The structures of the complexes confirm the bisubstrate character of the inhibitors by revealing the signature binding modes of the moieties targeting the ATP-binding site and the protein substrate-binding site of the kinase. This is the first structural model of a bisubstrate inhibitor targeting haspin. The presented structural data represent a model for the future development of more specific haspin inhibitors. Co-crystal structures of the protein kinase haspin with bisubstrate inhibitors.,Lavogina D, Kestav K, Chaikuad A, Heroven C, Knapp S, Uri A Acta Crystallogr F Struct Biol Commun. 2016 May 1;72(Pt 5):339-45. doi:, 10.1107/S2053230X16004611. Epub 2016 Apr 22. PMID:27139824[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Categories: Homo sapiens | Large Structures | Synthetic construct | Arrowsmith CH | Bountra C | Chaikuad A | Edwards AM | Heroven C | Kestav K | Knapp S | Lavogina D | Uri A | Von Delft F
