| Structural highlights
Function
FKS1_YEAST Alternate catalytic subunit of the 1,3-beta-glucan synthase (GS) complex. Synthesizes 1,3-beta-glucan, a major structural component of the yeast cell wall. Involved in cell wall synthesis, maintenance and remodeling.[1]
Publication Abstract from PubMed
1,3-beta-Glucan serves as the primary component of the fungal cell wall and is produced by 1,3-beta-glucan synthase located in the plasma membrane. This synthase is a molecular target for antifungal drugs such as echinocandins and the triterpenoid ibrexafungerp. In this study, we present the cryo-electron microscopy structure of Saccharomyces cerevisiae 1,3-beta-glucan synthase (Fks1) at 2.47-A resolution. The structure reveals a central catalytic region adopting a cellulose synthase fold with a cytosolic conserved GT-A-type glycosyltransferase domain and a closed transmembrane channel responsible for glucan transportation. Two extracellular disulfide bonds are found to be crucial for Fks1 enzymatic activity. Through structural comparative analysis with cellulose synthases and structure-guided mutagenesis studies, we gain previously unknown insights into the molecular mechanisms of fungal 1,3-beta-glucan synthase.
Structure of a fungal 1,3-beta-glucan synthase.,Zhao CR, You ZL, Chen DD, Hang J, Wang ZB, Ji M, Wang LX, Zhao P, Qiao J, Yun CH, Bai L Sci Adv. 2023 Sep 15;9(37):eadh7820. doi: 10.1126/sciadv.adh7820. Epub 2023 Sep , 13. PMID:37703377[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Utsugi T, Minemura M, Hirata A, Abe M, Watanabe D, Ohya Y. Movement of yeast 1,3-beta-glucan synthase is essential for uniform cell wall synthesis. Genes Cells. 2002 Jan;7(1):1-9. PMID:11856368 doi:10.1046/j.1356-9597.2001.00495.x
- ↑ Zhao CR, You ZL, Chen DD, Hang J, Wang ZB, Ji M, Wang LX, Zhao P, Qiao J, Yun CH, Bai L. Structure of a fungal 1,3-β-glucan synthase. Sci Adv. 2023 Sep 15;9(37):eadh7820. PMID:37703377 doi:10.1126/sciadv.adh7820
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