1zib
From Proteopedia
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| , resolution 2.0Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
REDUCED PSEUDOAZURIN
Overview
New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress.
About this Structure
1ZIB is a Single protein structure of sequence from Achromobacter cycloclastes. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013., Inoue T, Nishio N, Kai Y, Harada S, Ohshiro Y, Suzuki S, Kohzuma T, Shidara S, Iwasaki H, J Biochem. 1993 Dec;114(6):761-2. PMID:8138527
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