2kau
From Proteopedia
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | UREC (Klebsiella aerogenes), UREB (Klebsiella aerogenes), UREA (Klebsiella aerogenes) | ||||||
| Activity: | Urease, with EC number 3.5.1.5 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION
Overview
The crystal structure of urease from Klebsiella aerogenes has been determined at 2.2 A resolution and refined to an R factor of 18.2 percent. The enzyme contains four structural domains: three with novel folds playing structural roles, and an (alpha beta)8 barrel domain, which contains the bi-nickel center. The two active site nickels are 3.5 A apart. One nickel ion is coordinated by three ligands (with low occupancy of a fourth ligand) and the second is coordinated by five ligands. A carbamylated lysine provides an oxygen ligand to each nickel, explaining why carbon dioxide is required for the activation of urease apoenzyme. The structure is compatible with a catalytic mechanism whereby urea ligates Ni-1 to complete its tetrahedral coordination and a hydroxide ligand of Ni-2 attacks the carbonyl carbon. A surprisingly high structural similarity between the urease catalytic domain and that of the zinc-dependent adenosine deaminase reveals a remarkable example of active site divergence.
About this Structure
2KAU is a Protein complex structure of sequences from Klebsiella aerogenes. Full crystallographic information is available from OCA.
Reference
The crystal structure of urease from Klebsiella aerogenes., Jabri E, Carr MB, Hausinger RP, Karplus PA, Science. 1995 May 19;268(5213):998-1004. PMID:7754395
Page seeded by OCA on Mon Mar 31 04:03:06 2008
