This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2nuo
From Proteopedia
| |||||||
| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Related: | 2gty, 2gux, 2gue, 2guc, 2gud, 2hyr, 2hyq, 2i43
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a complex of griffithsin with glucose
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
About this Structure
2NUO is a Single protein structure of sequence from Griffithsia. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736
Page seeded by OCA on Mon Mar 31 04:08:46 2008
