2pt7
From Proteopedia
| |||||||
| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | cag-alfa, cag-alpha (Helicobacter pylori), HP1451 (Helicobacter pylori) | ||||||
| Related: | 1G6O, 1OPX, 1NLY, 1NLZ, 2GZA
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Cag VirB11 (HP0525) and an inhibitory protein (HP1451)
Overview
Helicobacter pylori is one of the world's most successful human pathogens causing gastric ulcers and cancers. A key virulence factor of H. pylori is the Cag pathogenicity island, which encodes a type IV secretion system. HP0525 is an essential component of the Cag system and acts as an inner membrane associated ATPase. HP0525 forms double hexameric ring structures, with the C-terminal domains (CTDs) forming a closed ring and the N-terminal domains (NTDs) forming a dynamic, open ring. Here, the crystal structure of HP0525 in complex with a fragment of HP1451, a protein of previously unknown function, is reported. The HP1451 construct consists of two domains similar to nucleic acid-binding domains. Two HP1451 molecules bind to the HP0525 NTDs on opposite sides of the hexamer, locking it in the closed form and forming a partial lid over the HP0525 chamber. From the structure, it is suggested that HP1451 acts as an inhibitory factor of HP0525 to regulate Cag-mediated secretion, a suggestion confirmed by results of in vitro ATPase assay and in vivo pull-down experiments.
About this Structure
2PT7 is a Protein complex structure of sequences from Helicobacter pylori. Full crystallographic information is available from OCA.
Reference
Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase., Hare S, Fischer W, Williams R, Terradot L, Bayliss R, Haas R, Waksman G, EMBO J. 2007 Nov 28;26(23):4926-34. Epub 2007 Nov 1. PMID:17972918
Page seeded by OCA on Mon Mar 31 04:40:18 2008
