User:Boris Brumshtein
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CV
Personal Information
Name: Boris Brumshtein
Languages: English, Hebrew and Russian
Current residence: Rehovot, Israel.
Contact Information
Department of Structural Biology, Weizmann Institute of Science Rehovot, 76100, Israel
E-mail:
Tell: +972-52-6902631 (private) +972-8-9342647 (work)
Education
2003 - present Direct PhD program at the Weizmann Institute of Science, Faculty of Chemistry, Department of Structural Biology. Estimated graduation - summer 2008. Scientific advisors: Prof. Joel L. Sussman, Prof. Israel Silman, Prof. Anthony H. Futerman Thesis topic: "Structural and biochemical studies on acid-beta-glucosidase, the defected protein in Gaucher disease".
2002-2003 MSc student, Weizmann Institute of Science, Faculty of Chemistry, Department of Structural Biology. Scientific advisor: Prof. Joel Sussman.
1999-2002 BA in Biochemistry, Technion, Israel Institute of Science and Technology, Faculty of Chemistry, cum laude.
Qualifications
In my research I employ different protein biochemistry, enzymology and crystallography methods, thus I gained a lot of experience in the following techniques:
Crystallography
- Protein crystallization utilizing microbatch crystallization, IMPAX I-5, Oryx and Mosquito crystallization robots.
- Ab initio crystallization and crystals optimization. Various crystallization techniques, such as: soaking, co-crystallization, crystallization in presence of heavy metals for phasing, etc.
- Crystal handling and manipulations, micro-manipulations with ZEISS microscope/manipulator system.
- Data acquisition using Rigaku Raxis 4/4++ or synchrotron x-ray sources.
- X-ray data processing software: XDS, HKL2000.
- Protein structure determination using MR, SIR, MIR, MAD, SIRAS approaches with CNS, SHELX and CCP4 software suits.
- Molecular modeling, visualization and analysis software: PyMol, Coot, XtalView, O, CCP4, APBS.
- Docking and small molecule/drug database mining with AutoDock - drug docking and virtual screening, Cambridge Structure Database (CSB), ZINC, ChemOffice.
Molecular biology and biochemistry
- Protein cloning and expression in E.Coli.
- Biochemical and protein purification techniques: HPLC, FPLC, Ni-columns, Ion exchange columns, acryl-amide gels, size exclusion, affinity chromatography, gradient and analytical ultracentrifugation, ultra-filtration, Mini Prep cell, etc.
- Drug-Protein interaction analysis employing various structural, biochemical and biophysical assays such as: enzymatic assays, ELIZA, circular dichroism, dynamic light scattering, mass-spec, BiaCore, analytical ultracentrifugation, etc.
Programming languages
-Unix, C, Perl, Matlab, Java (basic programming).
The page is always under construction ; last update Boris Brumshtein 14:12, 8 June 2008 (IDT)
