1aw6

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Template:STRUCTURE 1aw6

GAL4 (CD), NMR, 24 STRUCTURES


Overview

We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex.

About this Structure

1AW6 is a Single protein structure of sequence from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entry 125d. Full crystallographic information is available from OCA.

Reference

Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination., Baleja JD, Thanabal V, Wagner G, J Biomol NMR. 1997 Dec;10(4):397-401. PMID:9460244 Page seeded by OCA on Fri May 2 10:46:08 2008

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