1c8c
From Proteopedia
CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS
Overview
Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.
About this Structure
1C8C is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116 Page seeded by OCA on Fri May 2 12:27:09 2008
Categories: Single protein | Sulfolobus solfataricus | Edmondson, S P. | Gao, Y G. | Liaw, Y C. | Robinson, H. | Shriver, J W. | Su, S. | Wang, A H.J. | Achaeabacteria | Dna binding protein | Electrostatic | Hyperthermophile | Molecular modeling | Protein stability | Protein-dna interaction | T-g mismatch | X-ray crystallography
