1h0h
From Proteopedia
TUNGSTEN CONTAINING FORMATE DEHYDROGENASE FROM DESULFOVIBRIO GIGAS
Overview
Desulfovibrio gigas formate dehydrogenase is the first representative of a tungsten-containing enzyme from a mesophile that has been structurally characterized. It is a heterodimer of 110 and 24 kDa subunits. The large subunit, homologous to E. coli FDH-H and to D. desulfuricans nitrate reductase, harbors the W site and one [4Fe-4S] center. No small subunit ortholog containing three [4Fe-4S] clusters has been reported. The structural homology with E. coli FDH-H shows that the essential residues (SeCys158, His159, and Arg407) at the active site are conserved. The active site is accessible via a positively charged tunnel, while product release may be facilitated, for H(+) by buried waters and protonable amino acids and for CO(2) through a hydrophobic channel.
About this Structure
1H0H is a Protein complex structure of sequences from Desulfovibrio gigas. Full crystallographic information is available from OCA.
Reference
Gene sequence and the 1.8 A crystal structure of the tungsten-containing formate dehydrogenase from Desulfovibrio gigas., Raaijmakers H, Macieira S, Dias JM, Teixeira S, Bursakov S, Huber R, Moura JJ, Moura I, Romao MJ, Structure. 2002 Sep;10(9):1261-72. PMID:12220497 Page seeded by OCA on Fri May 2 18:15:20 2008
