1jf9
From Proteopedia
Crystal Structure of selenocysteine lyase
Overview
The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.
About this Structure
1JF9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487 Page seeded by OCA on Fri May 2 21:09:13 2008
Categories: Escherichia coli | Selenocysteine lyase | Single protein | Burley, S K. | Lima, C D. | NYSGXRC, New York Structural GenomiX Research Consortium. | Cysteine | New york structural genomix research consortium | Nif | Nysgxrc | Protein structure initiative | Psi | Pursulfide | Selenocysteine | Structural genomic
