1n7a
From Proteopedia
RIP-Radiation-damage Induced Phasing
Overview
The use of third generation synchrotron sources has led to renewed concern about the effect of ionizing radiation on crystalline biological samples. In general, the problem is seen as one to be avoided. However, in this paper, it is shown that, far from being a hindrance to successful structure determination, radiation damage provides an opportunity for phasing macromolecular structures. This is successfully demonstrated for both a protein and an oligonucleotide, by way of which complete models were built automatically. The possibility that, through the exploitation of radiation damage, the phase problem could become less of a barrier to macromolecular crystal structure determination is discussed.
About this Structure
Full crystallographic information is available from OCA.
Reference
Specific radiation damage can be used to solve macromolecular crystal structures., Ravelli RB, Leiros HK, Pan B, Caffrey M, McSweeney S, Structure. 2003 Feb;11(2):217-24. PMID:12575941 Page seeded by OCA on Sat May 3 02:11:01 2008
Categories: Caffrey, M. | Leiros, H K.S. | McSweeney, S. | Pan, B. | Ravelli, R B.G. | Dna | Rna
