357d
From Proteopedia
3.5 A STRUCTURE OF FRAGMENT I FROM E. COLI 5S RRNA
Overview
Two new RNA structures portray how non-Watson-Crick base pairs and metal ions can produce a unique RNA shape suitable for recognition by proteins. The crystal structures of a 62 nt domain of E. coli 5S ribosomal RNA and a duplex dodecamer encompassing an internal loop E have been determined at 3.0 and 1.5 A, respectively. This loop E region is distorted by three "cross-strand purine stacks" and three novel, water-mediated noncanonical base pairs and stabilized by a four metal ion zipper. These features give its minor groove a unique hydrogen-bonding surface and make the adjacent major groove wide enough to permit recognition by the ribosomal protein L25, which is expected to bind to this surface.
About this Structure
Full crystallographic information is available from OCA.
Reference
Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain., Correll CC, Freeborn B, Moore PB, Steitz TA, Cell. 1997 Nov 28;91(5):705-12. PMID:9393863 Page seeded by OCA on Sun May 4 20:17:02 2008
Categories: Correll, C C. | Freeborn, B. | Moore, P B. | Steitz, T A. | Double helix | Internal loop | Kinked | Mismatched | Modified | Overhanging base | U-rna
