1s10
From Proteopedia
Snapshots of replication through an abasic lesion: structural basis for base substitution and frameshift
Overview
Dpo4 from S. Solfataricus, a DinB-like Y family polymerase, efficiently replicates DNA past an abasic lesion. We have determined crystal structures of Dpo4 complexed with five different abasic site-containing DNA substrates and find that translesion synthesis is template directed with the abasic site looped out and the incoming nucleotide is opposite the base 5' to the lesion. The ensuing DNA synthesis generates a -1 frameshift when the abasic site remains extrahelical. Template realignment during primer extension is also observed, resulting in base substitutions or even +1 frameshifts. In the case of a +1 frameshift, the extra nucleotide is accommodated in the solvent-exposed minor groove. In addition, the structure of an unproductive Dpo4 ternary complex suggests that the flexible little finger domain facilitates DNA orientation and translocation during translesion synthesis.
About this Structure
1S10 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Snapshots of replication through an abasic lesion; structural basis for base substitutions and frameshifts., Ling H, Boudsocq F, Woodgate R, Yang W, Mol Cell. 2004 Mar 12;13(5):751-62. PMID:15023344 Page seeded by OCA on Sat May 3 08:09:35 2008
