3brf
From Proteopedia
CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221
Overview
The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
About this Structure
3BRF is a Protein complex structure of sequences from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:18381292 Page seeded by OCA on Thu Apr 24 09:33:19 2008
Categories: Caenorhabditis elegans | Protein complex | Kovall, R A. | Wilson, J J. | Ank repeat | Developmental protein | Differentiation | Dna binding protein/dna complex | Dna-binding | Egf-like domain | Glycoprotein | Membrane | Notch | Protein-dna complex | Signaling | Transcription | Transmembrane
