1sau
From Proteopedia
The Gamma subunit of the dissimilatory sulfite reductase (DsrC) from Archaeoglobus fulgidus at 1.1 A resolution
Overview
The structure of the 115 amino-acid residue protein DsvC was determined based on the anomalous scattering provided by the five S atoms present in the structure. By collecting the diffraction data at a wavelength of 1.9 A, the anomalous signal provided by the S atoms was enhanced. However, significant radiation damage occurred during the course of the experiment, which led to differences between different parts of the data set. Only by dividing the total data set into five data sets was it possible to obtain phases; these could then be successfully extended to allow structure determination by the automated model-building program ARP/wARP. A computational correction for the radiation damage was found to significantly improve the success rate in determining the heavy-atom substructure and to improve phasing and refinement statistics.
About this Structure
1SAU is a Single protein structure of sequence from Archaeoglobus fulgidus dsm 4304. Full crystallographic information is available from OCA.
Reference
Determination of a novel structure by a combination of long-wavelength sulfur phasing and radiation-damage-induced phasing., Weiss MS, Mander G, Hedderich R, Diederichs K, Ermler U, Warkentin E, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):686-95. Epub 2004, Mar 23. PMID:15039557 Page seeded by OCA on Sat May 3 08:29:31 2008
