1r3g
From Proteopedia
1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues
Overview
[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.
About this Structure
Full crystallographic information is available from OCA.
Reference
2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796 Page seeded by OCA on Sat May 3 07:01:45 2008
Categories: Egli, M. | Lesnik, E. | Manoharan, M. | Prakash, T P. | Puschl, A. | Tereshko, V. | Dna
