3bwp
From Proteopedia
Crystal structure of a self-spliced group II intron
Overview
Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor.
About this Structure
3BWP is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Crystal structure of a self-spliced group II intron., Toor N, Keating KS, Taylor SD, Pyle AM, Science. 2008 Apr 4;320(5872):77-82. PMID:18388288 Page seeded by OCA on Wed Apr 16 23:13:05 2008
Categories: Single protein | Keating, K S. | Pyle, A M. | Taylor, S D. | Toor, N. | Group ii | Intron | Ribonucleic acid | Rna
