2o9u
From Proteopedia
Monellin (MNEI) at 1.15 resolution
Overview
The X-ray crystal structure of a single-chain monellin protein (MNEI) has been determined at 1.15 A resolution. The model was refined to convergence employing anisotropic displacement parameters and riding H atoms to produce a final model with R(work) and R(free) values of 0.132 and 0.162, respectively. The crystal contains a single MNEI protein in the asymmetric unit and unusually lacks the dimer interface observed in all previous crystal structures of monellin and its single-chain derivatives. The high resolution allowed a more detailed view of MNEI than previously possible, with 38 of the 96 residues modelled with alternative side-chain conformations, including four core residues Thr12, Cys41, Leu62 and Ile75. Four stably bound negative ions were also located, providing new insight into potential electrostatic interactions of MNEI with the largely negatively charged surface of the sweet taste receptor T1R2-T1R3.
About this Structure
2O9U is a Single protein structure of sequence from Dioscoreophyllum cumminsii. Full crystallographic information is available from OCA.
Reference
Monellin (MNEI) at 1.15 A resolution., Hobbs JR, Munger SD, Conn GL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):162-7. Epub 2007 Feb 13. PMID:17329805 Page seeded by OCA on Wed Jun 18 12:16:24 2008
