2of6
From Proteopedia
Structure of immature West Nile virus
Overview
The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.
About this Structure
2OF6 is a Single protein structure of sequence from West nile virus. Full crystallographic information is available from OCA.
Reference
Structure of immature West Nile virus., Zhang Y, Kaufmann B, Chipman PR, Kuhn RJ, Rossmann MG, J Virol. 2007 Jun;81(11):6141-5. Epub 2007 Mar 21. PMID:17376919 Page seeded by OCA on Sun May 4 10:46:48 2008
