1rld
From Proteopedia
SOLID-STATE PHASE TRANSITION IN THE CRYSTAL STRUCTURE OF RIBULOSE 1,5-BIPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE
Overview
The crystal structure is described of ribulose 1,5-bisphosphate carboxylase/oxygenase in a new crystal form. This new form (form V) was obtained from a previously known crystal form (form III) through a solid-state phase transition. The solid-state phase transition was brought about by transferring the crystal from a high-salt low-pH mother liquor to a low-salt high-pH synthetic mother liquor. The interplay of electrostatic repulsion and osmotic pressure induced a unit-cell shrinkage of 24 A along the c axis and expansion of 4 A along the a and b axes. The space group also changed from I422 to I4. The new crystal form was shown to be more resistant to X-ray radiation damage, which suggests the effect of crystal stabilization by non-penetrating molecules. The structure of ribulose 1,5-bisphosphate carboxylase/oxygenase in the new crystal form is compared with that of the old crystal form.
About this Structure
1RLD is a Protein complex structure of sequences from Nicotiana tabacum. Full crystallographic information is available from OCA.
Reference
Solid-state phase transition in the crystal structure of ribulose 1,5-bisphosphate carboxylase/oxygenase., Zhang KY, Eisenberg D, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):258-62. PMID:15299436 Page seeded by OCA on Sat May 3 07:38:25 2008
