1zlb

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Template:STRUCTURE 1zlb

Crystal structure of catalytically-active phospholipase A2 in the absence of calcium


Overview

The electrophile Ca(2+) is an essential multifunctional co-factor in the phospholipase A(2) mediated hydrolysis of phospholipids. Crystal structures of an acidic phospholipase A(2) from the venom of Bothrops jararacussu have been determined both in the Ca(2+) free and bound states at 0.97 and 1.60 A resolutions, respectively. In the Ca(2+) bound state, the Ca(2+) ion is penta-coordinated by a distorted pyramidal cage of oxygen and nitrogen atoms that is significantly different to that observed in structures of other Group I/II phospholipases A(2). In the absence of Ca(2+), a water molecule occupies the position of the Ca(2+) ion and the side chain of Asp49 and the calcium-binding loop adopts a different conformation.

About this Structure

1ZLB is a Single protein structure of sequence from Bothrops jararacussu. Full crystallographic information is available from OCA.

Reference

Insights into metal ion binding in phospholipases A2: ultra high-resolution crystal structures of an acidic phospholipase A2 in the Ca2+ free and bound states., Murakami MT, Gabdoulkhakov A, Genov N, Cintra AC, Betzel C, Arni RK, Biochimie. 2006 May;88(5):543-9. Epub 2005 Dec 5. PMID:16376474 Page seeded by OCA on Sat May 3 17:46:00 2008

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